Protocols

Synthesis of CMRD Iron Oxide Particles

Mammalian Cell Culture

• Recommended Medium Volumes for Dishes

• Cell Freezing

• Cell Thawing

• Passaging Cells

• Culture Medium and Reagents

AAV

• AAV Transfection

\ * AAV Harvesting

• AAV - Determining Infectious Titer

Rhinovirus

PFU Assay

Purification of HRV16

Protein techniques

FITC Labeling of Proteins

Protein G Conjugation to CMRD-SPIO

Purification of ICAM-1 DI

SDS-PAGE

Western Blotting

Ethidium Bromide Disposal