https://www.jin-lab.org/ 2026-04-29T15:18:02+00:00 https://www.jin-lab.org/ https://www.jin-lab.org/lib/tpl/bootstrap3/images/favicon.ico text/html 2021-09-29T14:34:44+00:00 Anonymous (anonymous@undisclosed.example.com) https://www.jin-lab.org/doku.php?id=resources:protocols:index&rev=1632918884&do=diff Protocols (Under construction) Synthesis of CMRD Iron Oxide Particles Mammalian Cell Culture * Recommended Medium Volumes for Dishes * Cell Freezing * Cell Thawing * Passaging Cells * Culture Medium and Reagents Rhinovirus PFU Assay Purification of HRV16 Protein techniques FITC Labeling of Proteins Protein G Conjugation to CMRD-SPIO Purification of ICAM-1 DI SDS-PAGE Western Blotting Ethidium Bromide Disposal Streptavidin Culturing Streptavidin Animal protocol Mou… text/html 2011-04-20T15:16:29+00:00 Anonymous (anonymous@undisclosed.example.com) https://www.jin-lab.org/doku.php?id=resources:protocols:mouse_protocol_2009-0071&rev=1303305389&do=diff Jin Lab Mouse protocol 2009-0071 [:protocols:mouse_protocol_jin_moosoo_2009-0071_as_of_april_13_2011_.pdf] Created: 04/20/2011 Yogindra Vedvyas text/html 2011-02-25T22:56:12+00:00 Anonymous (anonymous@undisclosed.example.com) https://www.jin-lab.org/doku.php?id=resources:protocols:recommended_medium_volumes_for_dishes&rev=1298670972&do=diff Last Updated 11/1/07 MDR MEDIUM The recommended ratio of medium volume to surface area for monolayer cell cultures is 0.2-0.3 ml/cm2 *. The upper limit is set by gaseous diffusion through the liquid; the optimum ratio depends on the oxygen requirement of the cells. Cells with a high oxygen requirement grow better in shallow medium (~2mm), those with a low requirement grow better in deep medium (~5mm). Exceeding a medium depth of 5mm can hinder gas exchange and impair growth. text/html 2011-12-14T22:58:44+00:00 Anonymous (anonymous@undisclosed.example.com) https://www.jin-lab.org/doku.php?id=resources:protocols:sds-page&rev=1323899924&do=diff SDS-PAGE Pouring SDS-Polyacrylamide gels Reagents * 30% acrylamide mix * * 1.5M Tris (pH 8.8) * 1.0M Tris (pH 6.8) * 10% SDS * 10% Ammonium persulfate (made up directly before use) * TEMED *unpolymerized acrylamide is a neurotoxin – use gloves text/html 2011-10-28T18:33:51+00:00 Anonymous (anonymous@undisclosed.example.com) https://www.jin-lab.org/doku.php?id=resources:protocols:western_blotting&rev=1319819631&do=diff Western Blotting Run SDS-PAGE as described in SDS-PAGE protocol Transferring proteins to membrane * Once SDS-PAGE is finished, trim off wells and stacking gel with a razor blade, cut the bottom left corner of the gel * Cut a piece of pvdf to the size of the gel to be transferred and cut the bottom left corner also. Pre-soak the pvdf* in 100% methanol for at least 5 minutes prior to use.